Use Of Plant Cell Suspension Extracellular Vesicle-Derived Small Rnas As Cosmetic Active Ingredient

Abstract

The present invention pertains to the method of obtaining a raw material with anti-aging, wound healing, spot-reducing, dark circle-reducing, and anti-hair loss activities for use in cosmetic products. The invention comprises the steps of obtaining H. cordata cell suspensions, adding 0.05-0.5 mM MeJa to the H. cordata cell suspension culture medium on the 12th-18th day of the culture, incubating the culture with MeJA for 60-80 hours, isolating extracellular vesicles (EVs) from the culture medium after incubation, isolating small RNAs from EVs, and encapsulating the EV-derived small RNAs in cationic liposomes. The invention covers the use of individual or combinations of plants, including Houttuynia cordata, Centella asiatica, Cydonia oblonga, Malva sylvestris, Persea americana, Rosa damascena, Helichrysum arenarium, Glycine max, Prunella vulgaris, Symphytum officinale, Malva sylvestris, Scutellaria baicalensis, Pelargonium graveolens, Filipendula ulmaria, Passiflora edulis, Marribium vulgare, Linum tauricum, Lycium barbarum, Papaver rhoeas, Paeonia peregrina, Leontopodium alpinum, Prunus dulcis, Inula helenium, and Psoralea corylifolia, as the plant in the invention.

Dependencies are inferred from the claim text.