Novel Temperature-Optimized Bacilli
AssigneeChr. Hansen A/S
InventorsPatricia Dominguez CUEVAS, Raquel Azevedo, Karin Bjerre, Iuliana Nita, Rute Neves, Lars Moelbak
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Abstract
The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.
Claims20
A kit comprising:
- (a) a Bacillus paralicheniformis having a mutation in its closest ortholog of the bioF gene found in the strain deposited at Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig as accession number DSM 31110 (B. paralicheniformis DSM 31110);
- (b) instructions for applying said Bacillus paralicheniformis to a fertilizer and/or a plant seed and/or a plant growth medium and/or a plant.
A method comprising applying a Bacillus paralicheniformis to a fertilizer and/or a plant seed and/or a plant growth medium and/or a plant, said Bacillus paralicheniformis having a mutation in its closest ortholog of the bioF gene found in the strain deposited at Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig as accession number DSM 31110 (B. paralicheniformis DSM 31110).
A method comprising:
- (a) providing a plurality of Bacillus candidate strains;
- (b) detecting, in each Bacillus candidate strain, the presence or absence of:
- i. a mutation in the closest ortholog of the hrcA gene of DSM 31110, optionally a loss-of-function mutation, optionally a nonsense mutation that introduces a premature stop codon;
- and/or
- ii. a mutation in the closest ortholog of the bioF gene of DSM 31110, optionally a missense mutation, optionally missense mutation that produces an amino acid substitution at a position corresponding to Arg37, Ala64, Ala244, Asp300 or Pro314 of SEQ ID NO:
- 4, optionally a missense mutation that produces an amino acid substitution, relative to SEQ ID NO:
- 4, selected from Arg37Trp, Ala64Val, Ala244Val, Asp300Gly and Pro314Ser;
- (c) selecting a Bacillus candidate strain comprising the mutation of step (b)(i) and/or step (b)(ii).
Dependencies are inferred from the claim text.