Genetically modified pig production method, established somatic cell, and raw material for producing genetically modified pig

Abstract

The purpose of the present invention is to provide: a method for producing a genetically modified pig having, introduced selectively to a tissue or organ, a gene group for expressing a specific functional protein in the tissue or organ; somatic cells established therefrom; and a raw material for producing a genetically modified pig. Provided is a method for producing a genetically modified pig, the method comprising a step for introducing, into a safe harbor in a first gene-transfected cell, a gene cassette which includes a gene encoding a functional protein and includes a construct that enables the functional protein to be expressed by a recombinant enzyme. The first gene-introduced cell is a gene-introduced cell established from a gene-introduced pig that was gene-introduced by microfertilizing an egg with a liquid including a vector and pig sperm. The vector includes a promoter for causing expression in a specific tissue or organ, and a gene that encodes the recombinant enzyme that is downstream of and under the control of the promoter for causing expression in a specific tissue or organ.

Dependencies are inferred from the claim text.