Methods of producing chocolate from cacao plant cell materials
AssigneeCalifornia Cultured Inc
InventorsSteven William STEARNS, Rio Angelo STAMLER, Michael VILLANUEVA, Alan PERLSTEIN
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Abstract
The present invention relates to methods of producing chocolate, chocolate-like or food products, or food materials, from plant cell culture somatic embryogenesis biomass. The methods include culturing an in vitro cacao plant cell biomass, optionally with at least one microorganism and/or at least one enzyme, wherein the biomass comprises somatic embryos (SEs) from Theobroma cacao L. explant material. Chocolate, chocolate-like and food products obtained from such methods are also provided.
Claims24
A method of producing chocolate, chocolate-like products, food products, or food materials, based on in vitro cacao plant cell biomass, comprising the steps of:
- a) culturing an in vitro cacao plant cell biomass in a cell culture medium, optionally with at least one microorganism and/or at least one enzyme, wherein the biomass comprises somatic embryos from Theobroma cacao L. explant material;
- b) separating the plant cell biomass from the cell culture medium;
- c) optionally drying the biomass from step b) at least partially to obtain an at least partially dried biomass;
- d) optionally roasting the biomass from step b) or at least partially dried biomass from step c) to obtain a roasted biomass;
- e) optionally grinding the biomass from step b), the at least partially dried biomass from step c) or the roasted biomass from step d) to obtain a ground material;
- f) optionally conching the ground material from step e) to obtain a conched material;
- and
- g) optionally tempering the ground material from step e) or the conched material from step f).
(canceled)
Chocolate, chocolate-like products, food products or food materials, comprising somatic embryos (SE) from Theobroma cacao L. explant material produced through at least three successive cycles of direct and/or indirect somatic embryogenesis.
Dependencies are inferred from the claim text.