Microbeads with ratiometric lanthanide encoding for drug screening
AssigneesCZ Biohub SF, LLC, The Board of Trustees of the Leland Stanford Junior University, University of Copenhagen
InventorsPolly M. Fordyce, Jamin B. Hein
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Abstract
The disclosure provides methods and compositions for identifying agents that modulate binding of a protein to a peptide binding partner using spectrally encoded microbeads comprising binding site peptides in which there is a 1:1 linkage between sequences of the binding site peptide and the embedded spectral code.
Claims18
A method of identifying an agent that modulates interaction of a protein of interest with a binding site peptide, the method comprising providing a population of microbead-bound peptides comprising microbeads having distinguishable spectral codes, wherein a first microbead having a specific spectral code comprises a peptide having a first sequence that binds to the protein of interest, and a microbead having a different spectral code comprises a peptide having a second sequence that binds to the protein of interest, wherein the second sequence differs from the first sequence;
- contacting the population of microbeads with the protein of interest and a candidate agent for modulating binding of the protein interest to peptides present on the microbeads;
- detecting binding of the protein of interest to peptides on individual beads;
- and
- determining the spectral signature of individual microbeads, thereby determining the identity of the peptide linked to each bead.
An array comprising populations of lanthanide-encoded microbeads, each from the same library of microbeads, distributed into individual compartments, wherein each population comprises microbeads having distinguishable lanthanide spectral codes, wherein each microbead comprises a peptide comprising a binding site to a protein of interest attached thereto and wherein each microbead having the same spectral code comprises a peptide comprising the same binding site sequence and microbeads having different spectral codes comprise peptides comprising sites that differ in sequence.
Dependencies are inferred from the claim text.