Nanobiosensor and preparation method thereof and virus antibody detection method using same
AssigneeNational Defense Medical University
InventorsSzu-Cheng Kuo, Yu-Lin Hsu, Der-Jiang Chiao
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Abstract
A nanobiosensor includes a virus-like particle and a luciferase. The virus-like particle is assembled by a structural protein of a virus. The luciferase forms a fusion protein with the structural protein of the virus and is displayed on the surface of the virus-like particle. The virus-like particle is capable of capturing specific antibodies against the virus, and the luciferase is analyzed through a luciferase immunoprecipitation system, which enables a rapid detection of the specific antibodies against the virus.
Claims19
A nanobiosensor, comprising:
- a virus-like particle assembled by a structural protein of a virus;
- and
- a luciferase forming a fusion protein with the structural protein of the virus and displayed on the surface of the virus-like particle,
- wherein the virus-like particle is capable of capturing specific antibodies against the virus, and the luciferase is analyzed through a luciferase immunoprecipitation system, which enables a rapid detection of the specific antibodies against the virus.
A preparation method of a nanobiosensor, characterized by inserting a luciferase gene into a structural protein gene of a virus, and using a baculovirus/mosquito system to express, assemble and secrete a virus-like particle, wherein an expressed luciferase protein and an expressed structural protein of the virus form a fusion protein displayed on a surface of the virus-like particle.
A detection method of virus antibodies, comprising steps of:
- (a) providing a nanobiosensor and magnetic beads for capturing human IgG or IgM, wherein the nanobiosensor comprises a virus-like particle assembled by a structural protein of a virus and a luciferase forming a fusion protein with the structural protein of the virus and displayed on the surface of the virus-like particle;
- (b) mixing the nanobiosensor, the magnetic beads and a sample;
- (c) washing the magnetic beads to remove non-specific bindings;
- and
- (d) adding a substrate of the luciferase and detecting a light signal.
Dependencies are inferred from the claim text.