Silver nanoparticle based electrochemical biosensor using screen printed carbon electrode

Abstract

Disclosed is an electrochemical biosensor using silver nanoparticles (AgNPs) and screen printed carbon electrodes (SPCEs) that may be used to detect Shiga toxin. The AgNPs are modified with antibodies with specificity to a desired antigen (e.g., Shiga toxin-1). The reference electrode of the SPCEs may be modified with bleach, such that the reference electrode does not significantly contribute to a background signal while measuring the concentration of AgNPs. Related methods can isolate modified AgNP bound antigen molecules, dissolve the modified AgNPs in solution, and detect a concentration of AgNPs using an electrochemical assay that employs the modified SPCE. This detected concentration of AgNPs can subsequently be used to determine the concentration of the target antigen, wherein the concentration of the AgNPs directly correlates to the concentration of the target antigen.

Dependencies are inferred from the claim text.