Fret biosensor recombinant proteins
AssigneeThe Regents of the University of California
InventorsXinchang HE, Michelle Susanne FREI, Jin ZHANG
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Abstract
Provided herein are, inter alia, FRET biosensor recombinant proteins, which provide quantitative measurement of biomolecule activity in living cells with high spatiotemporal resolution. The FRET biosensor recombinant proteins provided herein are, inter alia, useful for detecting a biomolecule and activity of a biomolecule in a cell.
Claims107
A recombinant protein covalently bound to a chemical fluorophore, said recombinant protein comprising a fluorescent protein domain bound to a biosensing protein domain and a haloalkane dehalogenase domain bound to said biosensing protein domain, wherein:
- said chemical fluorophore is covalently bound to said haloalkane dehalogenase domain;
- and
- said chemical fluorophore and said fluorescent protein domain are a Forster Resonance Energy Transfer (FRET) pair.
A recombinant protein covalently bound to a chemical fluorophore, said recombinant protein comprising a split fluorescent protein domain bound to a biosensing protein domain and a haloalkane dehalogenase domain bound to said biosensing protein domain, wherein:
- said chemical fluorophore is covalently bound to said haloalkane dehalogenase domain;
- said split fluorescent protein domain is one part of a fluorescent protein split pair that forms a reconstituted fluorescent protein upon binding of a cognate split fluorescent protein;
- and
- said chemical fluorophore and said reconstituted fluorescent protein domain are a Förster Resonance Energy Transfer (FRET) pair.
A recombinant protein comprising a fluorescent protein domain bound to a biosensing protein domain and a split haloalkane dehalogenase domain, wherein:
- said split haloalkane dehalogenase domain is one part of a haloalkane dehalogenase split pair that forms a reconstituted haloalkane dehalogenase protein upon binding of a cognate haloalkane dehalogenase split protein domain.
Dependencies are inferred from the claim text.