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US 2026/0235587 A1Published application
Compositions and methods for structural and molecular characterization of extracellular particles
AssigneeOhio State Innovation Foundation
InventorsEduardo REATEGUI, Truc NGUYEN KIM, Xilal RIMA
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Abstract
Disclosed are systems and methods related to the multiparametric characterization of extracellular particles (EPs). The systems and methods can use a micropatterned substrate defining alignment features to correlating optical and non-optical images, thereby enabling the concurrent analysis of phenotypical and morphological features of EPs.
Claims43
A method for multiparametric characterization of extracellular particles (EPs), the method comprising:
- contacting a sample comprising a population of EPs with a micropatterned substrate to adsorb a subpopulation of the EPs on an imaging surface of the substrate, wherein the micropatterned substrate defines an alignment feature disposed thereon,
- obtaining an optical image of the adsorbed and/or bound EPs with the alignment feature;
- obtaining a non-optical image of the adsorbed and/or bound EPs with the alignment feature;
- and
- correlating the optical image and the non-optical image using the alignment feature as a reference to form a consolidated feature set from the optical and non-optical images.
2 more elements
A system for the multiparametric characterization of extracellular particles (EPs), the system comprising:
- a micropatterned substrate having an imaging surface and defining an alignment feature disposed thereon, wherein the micropatterned substrate is structured to adsorb a subpopulation of EPs on the imaging surface from a sample,
- an optical imaging element positionable to collect optical images of adsorbed EP and the alignment feature;
- a non-optical imaging element positionable to collect non-optical images of adsorbed and/or bound EPs and the alignment feature;
- and
- a processor;
- and
- a memory having instructions stored thereon, wherein execution of the instructions by the processor causes the processor to:
- receive optical and non-optical images showing the subpopulation of adsorbed and/or bound EPs;
- and
- orient the optical and non-optical images using the alignment feature to form a consolidated feature set from the optical and non-optical images.
7 more elements
Dependencies are inferred from the claim text.