Compositions and methods related to aptamer-based sensors
AssigneeNorth Carolina State University
InventorsYi Xiao, Juan Canoura
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Abstract
The present disclosure provides compositions and methods related to aptamer-based sensors. In particular, the present disclosure provides aptamer-based sensors, and related detection assays, that are capable of binding fentanyl (and derivatives and analogs thereof) at nanomolar concentrations in biological samples in a manner that is rapid and specific.
Claims21
A single-stranded nucleic acid molecule capable of binding fentanyl, or a derivative or analog thereof, comprising a nucleic acid sequence that is at least 50% identical to the following:
- X1GCTTGGX2TAGGTCGGGX3TTGGGTX4CGGX5X6;
- wherein X1 is A, T, C, or G;
- X2 is A, T, C, or G;
- X3 is A, T, C, or G;
- X4 is A, T, C, or G;
- X5 is A, T, C, or G;
- and X6 is A, T, C, or G (SEQ ID NO:
- 1).
A single-stranded nucleic acid molecule capable of binding fentanyl, or a derivative or analog thereof, comprising a nucleic acid sequence that is at least 50% identical to the following:
- X1X2AATGGX3AGCATTGGTX4X5X6X7X8X9X10X1X12X13X14X15X16;
- wherein X1 is A, T, C, or G;
- X2 is A, T, C, or G;
- X3 is A, T, C, or G;
- X4 is A, T, C, or G;
- X5 is A, T, C, or G;
- X6 is A, T, C, or G;
- X7 is A, T, C, or G;
- X8 is A, T, C, or G;
- X9 is A, T, C, or G;
- X10 A, T, C, or G;
- X11 is A, T, C, or G;
- X12 is A, T, C, or G;
- X13 is A, T, C, or G;
- X14 is A, T, C, or G;
- X15 is A, T, C, or G;
- and X16 is A, T, C, or G (SEQ ID NO:
- 3).
A method of identifying a nucleic acid aptamer capable of binding fentanyl, or a derivative or analog thereof, the method comprising:
- (i) exposing a library of candidate aptamers to fentanyl, or a derivative or analog thereof;
- (ii) collecting candidate aptamers exhibiting rapid target association kinetics;
- and
- (iii) repeating steps (i) and (ii) for at least one addition round of selection to generate a plurality of high-affinity candidate aptamers.
21-23. (canceled)
Dependencies are inferred from the claim text.