Observation device and observation method
AssigneeHamamatsu Photonics K.K.
InventorsOsamu YASUHIKO, Kozo TAKEUCHI
Life of this patent
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Abstract
An observation apparatus includes a light source, a mirror, a condenser lens, an objective lens, a beam splitter, an imaging unit, and an analysis unit. The analysis unit irradiates an observation object with light along each of a plurality of light irradiation directions by changing an orientation of a reflection surface of the mirror, acquires an interference intensity image at a reference position for each of the plurality of light irradiation directions from the imaging unit, and obtains a phase differential image of the observation object by performing predetermined processing based on the acquired interference intensity images.
Claims16
An observation apparatus comprising:
- an interference intensity image acquisition unit configured to acquire an interference intensity image of an observation object irradiated with light along each of a plurality of light irradiation directions;
- a complex amplitude image generation unit configured to generate a complex amplitude image based on the interference intensity image for each of the plurality of light irradiation directions;
- a transmission matrix generation unit configured to generate, based on the complex amplitude image of each of the plurality of light irradiation directions, a transmission matrix Trr(rout;
- rin) which relates a wavefront of light at each position rin on a first plane in a case in which the observation object is not present to a wavefront of light at each position rout on a second plane, which is the same as the first plane, in a case in which the observation object is present;
- a complex differential interference image generation unit configured to generate, based on the transmission matrix Trr(rout;
- rin), a complex differential interference image by generating C(rout;
- rin), which is an element-wise product of Trr(rout, rin) and Trr(rout−δr;
- rin−δr), weighting C(rout;
- rin) by an amplitude of C(rout;
- rin) for each element, and obtaining a sum of weighted C(rout;
- rin) on the second plane for each position rin on the first plane, or on the first plane for each position rout on the second plane;
- and
- a phase differential image generation unit configured to generate a phase differential image based on the complex differential interference image.
An observation method comprising:
- performing an interference intensity image acquisition of acquiring an interference intensity image of an observation object irradiated with light along each of a plurality of light irradiation directions;
- performing a complex amplitude image generation of generating a complex amplitude image based on the interference intensity image for each of the plurality of light irradiation directions;
- performing a transmission matrix generation of generating, based on the complex amplitude image of each of the plurality of light irradiation directions, a transmission matrix Trr(rout;
- rin) which relates a wavefront of light at each position rin on a first plane in a case in which the observation object is not present to a wavefront of light at each position rout on a second plane, which is the same as the first plane, in a case in which the observation object is present;
- performing a complex differential interference image generation of generating, based on the transmission matrix Trr(rout;
- rin), a complex differential interference image by generating C(rout;
- rin), which is an element-wise product of Trr(rout;
- rin) and Trr(rout−δr;
- rin−δr), weighting C(rout;
- rin) by an amplitude of C(rout, rin) for each element, and obtaining a sum of weighted C(rout;
- rin) on the second plane for each position rin on the first plane, or on the first plane for each position rout on the second plane;
- and
- performing a phase differential image generation of generating a phase differential image based on the complex differential interference image.
Dependencies are inferred from the claim text.