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US 2026/0235509 A1Published application
Observation device and observation method
AssigneeHamamatsu Photonics K.K.
InventorsOsamu YASUHIKO, Kozo TAKEUCHI
Life of this patent
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Abstract
An observation apparatus includes a light source, a mirror, a condenser lens, an objective lens, a beam splitter, an imaging unit, and an analysis unit. The analysis unit irradiates an observation object with light along each of a plurality of light irradiation directions by changing an orientation of a reflection surface of the mirror, acquires an interference intensity image at a reference position for each of the plurality of light irradiation directions from the imaging unit, and obtains a phase differential image of the observation object by performing predetermined processing based on the acquired interference intensity images.
Claims16
1:
- An observation apparatus comprising:
- an interference intensity image acquisition unit configured to acquire an interference intensity image of an observation object irradiated with light along each of a plurality of light irradiation directions;
- a complex amplitude image generation unit configured to generate a complex amplitude image based on the interference intensity image for each of the plurality of light irradiation directions;
- a transmission matrix generation unit configured to generate, based on the complex amplitude image of each of the plurality of light irradiation directions, a transmission matrix Trr(rout;
- rin) which relates a wavefront of light at each position rin on a first plane in a case in which the observation object is not present to a wavefront of light at each position rout on a second plane, which is the same as the first plane, in a case in which the observation object is present;
- a complex differential interference image generation unit configured to generate, based on the transmission matrix Trr(rout;
- rin), a complex differential interference image by obtaining a sum of element-wise products of Tr(rout;
- rin) and Trr*(rout−δr;
- rin−δr) on the second plane for each position rin on the first plane;
- and
- a phase differential image generation unit configured to generate a phase differential image based on the complex differential interference image.
8 more elements
2:
- The observation apparatus according to claim 1, wherein the interference intensity image acquisition unit is configured to acquire the interference intensity image of each of the plurality of light irradiation directions from an imaging unit configured to image the interference intensity image generated by interference between the light which irradiates the observation object along each of the plurality of light irradiation directions and passes through the observation object and reference light.
3:
- The observation apparatus according to claim 2, wherein the interference intensity image acquisition unit is configured to acquire the interference intensity image of each of the plurality of light irradiation directions from the imaging unit fer configured to image the interference intensity image when the observation object is irradiated with the light along each of the plurality of light irradiation directions in which positions of wavenumber vectors representing the light irradiation directions in a wavenumber space are discretely and periodically distributed;
- and
- the complex differential interference image generation unit is configured to generate the complex differential interference image by obtaining the sum of the products in each of a plurality of regions on the second plane, which are divided based on a periodic distribution of the positions of the wavenumber vectors respectively for the plurality of light irradiation directions in the wavenumber space.
4:
- The observation apparatus according to claim 1, wherein the interference intensity image acquisition unit is configured to acquire the interference intensity image generated by interference between the light which irradiates the observation object along each of the plurality of light irradiation directions and passes through the observation object and light which irradiates the observation object along a fixed light irradiation direction and passes through the observation object.
5:
- The observation apparatus according to claim 1, wherein the phase differential image generation unit is configured to
- generate the complex differential interference image by obtaining the sum of the element-wise products of Tr(rout;
- rin) and Trr*(rout−δr;
- rin−δr) on the second plane for each position rin on the first plane for a position relatively far from an imaging unit, and
- generate the complex differential interference image by obtaining the sum of the element-wise products of Tr(rout;
- rin) and Trr*(rout−δr;
- rin−δr) on the first plane for each position rout on the second plane for a position relatively close to the imaging unit.
4 more elements
6:
- The observation apparatus according to claim 1, wherein the phase differential image generation unit is configured to generate a three-dimensional phase differential image by generating the phase differential image at each position along an optical axis of an imaging unit.
7:
- The observation apparatus according to claim 6, further comprising a refractive index distribution image generation unit configured to generate a three-dimensional refractive index distribution image of the observation object based on the three-dimensional phase differential image.
8:
- An observation method comprising:
- performing an interference intensity image acquisition of acquiring an interference intensity image of an observation object irradiated with light along each of a plurality of light irradiation directions;
- performing a complex amplitude image generation of generating a complex amplitude image based on the interference intensity image for each of the plurality of light irradiation directions;
- performing a transmission matrix generation of generating, based on the complex amplitude image of each of the plurality of light irradiation directions, a transmission matrix Trr(rout;
- rin) which relates a wavefront of light at each position rin on a first plane in a case in which the observation object is not present to a wavefront of light at each position rout on a second plane, which is the same as the first plane, in a case in which the observation object is present;
- performing a complex differential interference image generation of generating, based on the transmission matrix Trr(rout;
- rin), a complex differential interference image by obtaining a sum of element-wise products of Trr(rout;
- rin) and Trr*(rout−δr;
- rin−δr) on the second plane for each position rin on the first plane;
- and
- performing a phase differential image generation of generating a phase differential image based on the complex differential interference image.
8 more elements
9:
- The observation method according to claim 8, wherein in the interference intensity image acquisition, the interference intensity image of each of the plurality of light irradiation directions is acquired from an imaging unit configured to image the interference intensity image generated by interference between the light which irradiates the observation object along each of the plurality of light irradiation directions and passes through the observation object and reference light.
10:
- The observation method according to claim 9, wherein in the interference intensity image acquisition, the interference intensity image of each of the plurality of light irradiation directions is acquired from the imaging unit configured to image the interference intensity image when the observation object is irradiated with the light along each of the plurality of light irradiation directions in which positions of wavenumber vectors representing the light irradiation directions in a wavenumber space are discretely and periodically distributed;
- and
- in the complex differential interference image generation, the complex differential interference image is generated by obtaining the sum of the products in each of a plurality of regions on the second plane, which are divided based on a periodic distribution of the positions of the wavenumber vectors respectively for the plurality of light irradiation directions in the wavenumber space.
11:
- The observation method according to claim 8, wherein in the interference intensity image acquisition, the interference intensity image generated by interference between the light which irradiates the observation object along each of the plurality of light irradiation directions and passes through the observation object and light which irradiates the observation object along a fixed light irradiation direction and passes through the observation object is acquired.
12:
- The observation method according to claim 8, wherein in the phase differential image generation,
- the complex differential interference image is generated by obtaining the sum of the element-wise products of Tr(rout;
- rin) and Trr*(rout−δr;
- rin−δr) on the second plane for each position rin on the first plane for a position relatively far from an imaging unit, and
- the complex differential interference image is generated by obtaining the sum of the element-wise products of Tr(rout;
- rin) and Trr*(rout−δr;
- rin−δr) on the first plane for each position rout on the second plane for a position relatively close to the imaging unit.
4 more elements
13:
- The observation method according to claim 8, wherein in the phase differential image generation, a three-dimensional phase differential image is generated by generating the phase differential image at each position along an optical axis of an imaging unit.
14:
- The observation method according to claim 13, further comprising performing a refractive index distribution image generation of generating a three-dimensional refractive index distribution image of the observation object based on the three-dimensional phase differential image.
15:
- A program for causing a computer to execute respective steps of the observation method according to claim 8.
16:
- A computer readable recording medium storing the program according to claim 15.