Methods for Determining a Data Filter for Detecting Particles of a Sample and Systems and Methods for Using Same

A method comprising:

• detecting light with a light detection system from particles of a sample in a flow stream;
• generating data signal waveforms in response to the detected light;
• and
• applying a data signal filter to the generated data signal waveforms, wherein the data signal filter is calculated based on a determined feature for data signals generated by the light detection system.

A system comprising:

• a light source configured to irradiate particles in a flow stream;
• a light detection system comprising a plurality of photodetectors;
• and
• a processor comprising memory operably coupled to the processor wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to:
• generate a data signal waveform in response to the detected light from a particle in the flow stream;
• determine a feature of the data signal waveform;
• and
• calculate a data signal filter from the determined feature of the data signal waveform.

A system comprising:

• a light source configured to irradiate particles of a sample in a flow stream;
• a light detection system comprising a plurality of photodetectors;
• and
• a processor comprising memory operably coupled to the processor wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to:
• generate data signal waveforms in response to the detected light;
• and
• apply a data signal filter to the generated data signal waveforms, wherein the data signal filter is calculated based on a determined feature for data signals generated by the light detection system.