Methods of selecting antibodies and antibody fragments
AssigneeAllele Biotechnology & Pharmaceuticals, Inc.
InventorsJiwu WANG, Gerard G. LAMBERT, Nathan C. SHANER
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Abstract
A multi-step method is disclosed for efficient selection of antibody or antibody fragments. In addition to the dramatically increased efficiency of finding antibodies against a given immunogen by the invented processes, the novel technology also differs from all previously known technologies in that the antibody or antibody fragments represent the most abundant antibodies induced by the immunogen, therefore having the highest potential of specificity and affinity. Utility of the invention can be found in virtually all areas that involve antibody or T cell receptor selection using any animal, against any immunogen.
Claims18
A method for generating pharmaceutical batches of immunogen-specific antibodies or antibody fragments comprising identifying and obtaining sequences of antibody variable region from genomic DNA or complementary DNA.
6:
- A method for identifying immunogen-specific antibody or antibody fragments through obtaining sequences of antibody variable region from genomic DNA or complementary DNA, antibody amino acid sequences, then combining the two sequence databases in identifying the specific antibodies.
7:
- A method of claim 6, wherein the antibody variable gene sequence information is from analyzing the antibody protein sequences that the V genes encode by MASS spectrometry.
8:
- A method of claim 7, wherein the antibody pool is prepared from serum of an immunized animal.
9:
- A method of claim 7, wherein the antibody pool is prepared by expressing antibody-encoding gDNA or cDNA pools in a host cell.
10:
- A method for presenting immunogens particularly suitable for sequence-based antibody selection which include:
- A) isolating cells from the host animal or an animal from the host species;
- 2) introducing polynucleotide that encode the immunogen intended for antibody generation;
- 3) collecting cells post transfection, then immunizing the host animal.
11:
- A method of claim 10, wherein the host cells are from llama.
12:
- A method of claim 10, wherein the polynucleotide is a cDNA.
13:
- A method of claim 10, wherein the polynucleotide is a gDNA.
A method of claim 9, wherein the polynucleotide is an mRNA.
A method for presenting immunogens suitable for pharmaceutical batch preparation comprising 1) sequence-based antibody selection and 2) transiently expressing high levels of immunogen gene by transfecting into modified mRNA mammalian cells as host cells.
A method for identifying antigen-specific antibody or antibody fragments through accumulating sequences of antibody variable regions and using software to establish antigen-antibody interactions, and using such a database to predict what antibody variable region would bind a given target.
Dependencies are inferred from the claim text.