Biosynthesis of 3-indoxyl phosphate from 3-hydroxyindole using kinases and indigo dyeing method using the same
AssigneeSeoul National University R&Db Foundation
InventorsByung Gee Kim, Sang Woo Seo, Jeongchan Lee
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Abstract
The present invention proposes an efficient production and dyeing method of an eco-friendly indigo precursor using indoxyl phosphate having a phosphoryl group as a functional group, thereby proposing a method for overcoming the problems of conventional indican and indoxyl acetates. To this end, the present invention found and characterized a novel kinase capable of producing 3-indoxyl phosphate from 3-hydroxyindole for the biosynthesis of 3-indoxyl phosphate, and also confirmed that when indigo denim fabric is dyed using indoxyl phosphate and alkaline phosphatase, a dyeing efficiency almost similar to that of chemical indigo dye is exhibited.
Claims22
A method for preparing 3-indoxyl phosphate, comprising reacting a kinase or a recombinant microbial strain expressing a kinase with 3-hydroxyindole.
A method for dyeing fabric, comprising the following steps:
- a) reacting a kinase or a recombinant microbial strain expressing a kinase with 3-hydroxyindole to produce 3-indoxyl phosphate;
- b) adding an alkaline phosphatase or a recombinant microbial strain expressing an alkaline phosphatase to the supernatant of the reaction mixture obtained in step a) to produce indigo;
- and
- c) dyeing a fabric by immersing the fabric in the supernatant of the reaction mixture obtained in step b).
Dependencies are inferred from the claim text.