Reduction in acetate produced by yeast with reduced expression of rsf2 or tda9
AssigneeDanisco US Inc.
InventorsCourtney Bement, Zhongqiang Chen, Xiaochun Fan, Daniel Joseph Macool, Min Qi, Yehong Jamie Wang, Zhixiong Xue, Quinn Qun Zhu
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Abstract
Described are compositions and methods relating to modified yeast with disrupted RSF2 or TDA9 genes. The yeast produces a decreased amount of acetate compared to otherwise identical parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate is an undesirable end product.
Claims16
Modified yeast cells derived from parental yeast cells, the modified yeast cells comprising a genetic alteration that causes the modified yeast cells to produce a decreased amount of one or more of RFS2 and/or TDA9 polypeptides compared to the parental yeast cells, wherein the modified yeast cells produce during fermentation a decreased amount of acetate compared to the amount of acetate produced by the parental yeast cells under identical fermentation conditions.
A method for decreasing the production of acetate from yeast cells grown on a carbohydrate substrate, comprising:
- introducing into parental yeast cells a genetic alteration that that decreases the production of functional RSF2 and/or TAD9 polypeptides compared to the amount produced in the parental cells.
Dependencies are inferred from the claim text.