Composition and method for the production of polyomaviral vector particles

A method for the production of polyomaviral vector particles, the method comprising the steps of:

• a) providing a first DNA construct comprising recombinant DNA and a polyomaviral intergenic region, wherein said first DNA construct does not comprise polyomaviral functional coding sequences and bacterial plasmid sequences;
• b) providing a cell line permissive for the wild type polyomavirus;
• c) introducing the first DNA construct of step a) into the cell line of step b);
• d) culturing the cell line obtained in step c) in a growth medium under conditions allowing the formation of polyomaviral vector particles;
• and
• e) harvesting the polyomaviral vector particles from the cell culture obtained in step d),
• wherein the method further comprises the step of introducing in step c) a second DNA construct capable of expressing functional polyomaviral capsid proteins, and wherein said second DNA construct cannot be encapsidated into the polyomaviral vector particles.