Modified promoter of a parthenogenesis gene
AssigneeKeygene N.V.
InventorsRik Hubertus Martinus OP DEN CAMP, Peter Johannes VAN DIJK
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Abstract
The invention provides a method to produce a mutant gene, wherein said gen comprises a modified promoter and wherein said gene is capable of inducing the parthenogenesis phenotype to a plant. The invention further provides said mutant gene, isolated nucleic acid molecule, construct or vector comprising the same. Also, the invention provides for a method to produce a parthenogenetic plant comprising the mutant gene, and the parthenogenetic plant thus obtained.
Claims21
-15. (canceled)
A method of producing a mutant gene capable of inducing a parthenogenesis phenotype, wherein said method comprises the steps of:
- (a) providing a gene comprising a sequence encoding a PAR protein of SEQ ID NO:
- 1, 6 or 11 or any orthologue thereof, operably linked to a promoter comprising one or more transcription factor MYB binding sites, wherein the PAR protein is characterized in that it comprises a zinc finger C2H2-type domain having the consensus amino acid sequence of SEQ ID NO:
- 37 and an EAR motif having the consensus amino acid sequence of SEQ ID NO:
- 58 or 59;
- and
- (b) modifying the promoter by modifying the sequence upstream of the one or more MYB binding sites to increase transcription of the encoded PAR protein.
Dependencies are inferred from the claim text.