Disease resistant melon plants
AssigneeSyngenta Crop Protection AG
InventorsAdrien MAUNY, Marc OLIVER, Bruno FONCELLE
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel melon plants displaying an increased resistance to Fusarium oxysporum f.sp. melonis race 1,2 infection. The present invention also relates to seeds and parts of said plants, for example fruits. The present invention further relates to methods of making and using such seeds and plants. The present invention also relates to novel genetic sequences associated with said increased resistance and to molecular markers associated with said novel genetic sequences.
Claims9
A method for identifying a cultivated melon plant, preferably a cultivated Cucumis melo plant, exhibiting resistance to FOM 1,2 and having at least one copy of said FOM 1,2 resistance-conferring introgressed sequence, said method comprising the step of detecting at least one of the following SNP markers:
- a) a G genotype in the heterozygous or homozygous state for SNP marker 1 at a position corresponding to position 95 in SEQ ID NO:
- 1;
- b) an A genotype in the heterozygous or homozygous state for SNP marker 2 at a position corresponding to position 113 in SEQ ID NO:
- 6;
- c) an A genotype in the heterozygous or homozygous state for SNP marker 3 at a position corresponding to position 73 in SEQ ID NO:
- 11;
- d) a G genotype in the heterozygous or homozygous state for SNP marker 4 at a position corresponding to position 239 in SEQ ID NO:
- 16;
- e) an A genotype in the heterozygous or homozygous state for SNP marker 5 at a position corresponding to position 163 in SEQ ID NO:
- 21;
- f) an A genotype in the heterozygous or homozygous state for SNP marker 6 at a position corresponding to position 59 in SEQ ID NO:
- 26;
- g) an A genotype in the heterozygous or homozygous state for SNP marker 7 at a position corresponding to position 119 in SEQ ID NO:
- 31;
- h) an A genotype in the heterozygous or homozygous state for SNP marker 8 at a position corresponding to position 69 in SEQ ID NO:
- 36;
- i) an A genotype in the heterozygous or homozygous state for SNP marker 9 at a position corresponding to position 136 in SEQ ID NO:
- 41;
- and/or
- j) a T genotype in the heterozygous or homozygous state for SNP marker 10 at a position corresponding to position 108 in SEQ ID NO:
- 46;
- thereby identifying a melon plant exhibiting resistance to FOM 1,2.
A method for assessing the genotype of a cultivated melon plant, preferably a cultivated Cucumis melo plant, exhibiting resistance to FOM 1,2, said method comprising the steps of:
- a) providing a sample from said plant, and,
- b) detecting in said sample a QTL locus located on chromosome 4 and associated with said FOM 1,2 resistance, said QTL locus being flanked by SNP markers 11 and 12, and at least one of the following SNP markers:
- i) a G genotype in the heterozygous or homozygous state for SNP marker 1 at a position corresponding to position 95 in SEQ ID NO:
- 1;
- ii) an A genotype in the heterozygous or homozygous state for SNP marker 2 at a position corresponding to position 113 in SEQ ID NO:
- 6;
- iii) an A genotype in the heterozygous or homozygous state for SNP marker 3 at a position corresponding to position 73 in SEQ ID NO:
- 11;
- iv) a G genotype in the heterozygous or homozygous state for SNP marker 4 at a position corresponding to position 239 in SEQ ID NO:
- 16;
- v) an A genotype in the heterozygous or homozygous state for SNP marker 5 at a position corresponding to position 163 in SEQ ID NO:
- 21;
- vi) an A genotype in the heterozygous or homozygous state for SNP marker 6 at a position corresponding to position 59 in SEQ ID NO:
- 26;
- vii) an A genotype in the heterozygous or homozygous state for SNP marker 7 at a position corresponding to position 119 in SEQ ID NO:
- 31;
- viii) an A genotype in the heterozygous or homozygous state for SNP marker 8 at a position corresponding to position 69 in SEQ ID NO:
- 36;
- ix) an A genotype in the heterozygous or homozygous state for SNP marker 9 at a position corresponding to position 136 in SEQ ID NO:
- 41;
- and/or
- x) a T genotype in the heterozygous or homozygous state for SNP marker 10 at a position corresponding to position 108 in SEQ ID NO:
- 46;
- and/or
- xi) any other DNA marker associated with said QTL locus flanked by SNP markers 11 and 12.
A method of identifying in a cultivated melon plant, preferably a cultivated Cucumis melo plant, an introgressed sequence associated with an increased resistance to FOM 1,2, said method comprising the step of detecting in said plant an allele of at least one DNA marker that is genetically linked to a QTL locus associated with said increased resistance to FOM 1,2, wherein said allele maps within 10 cM, preferably within 5 CM of said QTL locus located on chromosome 4 in a genomic region flanked by SNP markers 11 and 12.
A method of identifying a cultivated melon plant, preferably a cultivated Cucumis melo plant, exhibiting increased resistance to FOM 1,2 by identifying a QTL associated with said increased resistance to FOM 1,2, the method comprising the steps of:
- a) detecting at least one DNA marker from a melon plant, which DNA marker is linked to a chromosomal interval associated with increased resistance to FOM 1,2, wherein said chromosomal interval is flanked on each side by SNP markers having at least 80% sequence identity to SEQ ID NOs:
- 51 and 56;
- and
- b) identifying said melon plant comprising said at least one DNA marker.
A method of identifying a wild melon source of FOM 1,2 resistance trait on chromosome 4, comprising:
- a) providing a wild melon accession or a plurality of wild melon accessions,
- b) screening said wild melon accession or plurality of wild melon accessions using a kit which detects at least one of the SNP markers listed in Table 5, and,
- c) identifying a wild melon accession comprising said at least one SNP marker selected in the list of Table 5.
Use of any of SEQ ID NOs 1-60 for screening a population of melon plants for the presence of a QTL locus located on chromosome 4 and associated with an increased FOM 1,2 resistance.
Dependencies are inferred from the claim text.