System for regulating protein translation

Abstract

The present invention provides a system capable of translational regulation of a protein of interest specifically in accordance with the type of cell. The system for regulating protein translation contains the following mRNAs (a), (b), and (c), or DNAs encoding these mRNAs: (a) an mRNA that expresses a first fusion protein containing a first target-binding molecule specifically recognizing a first site of a target molecule, an N-terminal domain of a caged intein, and an N-terminal domain of a translational regulator; (b) an mRNA that expresses a second fusion protein containing a second target-binding molecule specifically recognizing a second site of the target molecule, a C-terminal domain of a caged intein, and a C-terminal domain of the translational regulator; and (c) a switch mRNA containing one or more binding motifs specifically recognizing the translational regulator, and a nucleic acid sequence encoding a protein of interest, and the translational regulator contains an RNA-binding protein.

Dependencies are inferred from the claim text.