ENGINEERING OF piRNA GENERATING SYNTHETIC RNAs FOR GREATER STABILITY AND SUBCELLULAR TARGETING
AssigneesThe University of Southern Mississippi, Arizona Board of Regents on Behalf of the University of Arizona
InventorsAlex Flynt, Judith Brown, Olga Mavrodi, Santosh Poudel
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Abstract
The present disclosure relates to synthetic RNAs comprising a piRNA sequence and a target sequence for a target gene of interest, vectors comprising the synthetic RNA, and genetically modified cells comprising the synthetic RNAs. Compositions and kits comprising the same are provided, as well as methods of regulating the expression of a target gene in an organism for pesticide treatment, control, or management. Also, transgenic plants containing the above-mentioned synthetic RNAs are made.
Claims23
A synthetic RNA for silencing a gene of a target species comprising:
- a piwi-interacting RNA (pi-RNA) sequence,
- a target sequence targeting the gene of the target species, and at least one of a)-b):
- a) one or more tetraloops appearing at at least one end of the synthetic RNA, and
- b) an RNA aptamer that binds to a receptor of a mitochondria of the target species,
- wherein the synthetic RNA induces silencing of the gene of the target species.
Dependencies are inferred from the claim text.