Modified bile salt hydrolase enzymes and methods of using the same
AssigneePurpose Bio, Inc.
InventorLital GILAD-SHAOULIAN
Life of this patent
Estimating the term…
Abstract
Disclosed are modified Christensenella minuta bile salt hydrolase (BSH) enzymes, prebiotics comprising the same, engineered bacterial cells comprising the modified BSH enzymes, probiotics comprising the engineered bacterial cells, methods of using the compositions to treat diseases and disorders, comprising the modified enzymes, and food products.
Claims73
A modified Christensenella minuta (C. minuta) bile salt hydrolase (BSH) enzyme at least 90% identical to SEQ ID NO:
- 1 and comprising one or more amino acid substitution at position C2, Y8, Y34, N46, H47, Y56, I57, Y64, P81, K90, F99, L127, S136, Y176, K189, S206, R207, A221, N292, or R323 relative to SEQ ID NO:
- 1.
A modified bile salt hydrolase (BSH) enzyme comprising amino acid substitutions at position I5V, Y8R, T9S, D11H, H12T, YT3F, R16M, L18Y, L20A, E21S, F22S, Y24E, N25G, T27K, V28I, T29V, V30I, T3TV, K33R, Y34K, F37L, H38K, F39L, R40S, K43E, L45I, N46S, H47N, M54I, Y56K, I57V, V58I, D60N, F61Y, Y64F, Y65K, D66M, T68C, S74A, G77A, L78I, N79S, D82G, N83I, D85S, K87G, V89P, K90T, E91P, Y93K, D94E, 196V, F99Y, F101L, W104Y, Q108R, A110G, S113D, R116L, I117E, E120K, Q121T, I122L, L124I, L127V, N128D, E131A, L133H, S136K, L138V, H139K, W140Y, Q145K, R146E, D147K, V151L, S153Q, F161Y, V165T, N170G, F174Y, M178L, K189N, E190K, T195K, A197S, A198P, E199S, L200I, E201K, Q204R, Y205I, R207S, A211N, L218Y, S220D, A221L, R223K, V225I, K226R, A228S, T230V, K231R, M232L, S234A, E240Y, S241D, S243Q, I244L, S245M, G252D, E255R, Q256N, Q257V, R258P, C260T, E265G, E269L, I270R, I272L, S274Q, S275T, C277I, N278D, K281R, Y284L, T287R, E290N, N292T, Y300N, E302C, N303D, D305N, N307D, T308K, S311E, Y312F, M315V, K316T, Q318P, N321Y, Y322E, R323L, N324K, and Y325K, relative to SEQ ID NO:
- 1.
A modified bile salt hydrolase (BSH) enzyme comprising amino acid substitutions at position I5V, Y8K, T9S, D11S, H12S, Y13W, R16M, L18Y, L20A, E21S, F22S, Y24E, T27E, V28I, T29V, V30I, T31V, K33R, Y34K, F37L, H38K, F39L, R40S, K43T, L45I, N46D, H47E, Y49N, M54I, Y56E, V58R, D60N, L63I, Y64F, Y65K, D66M, G77A, L78V, N79S, F80L, D82G, N83I, D85S, K87G, V89K, K90T, Y93K, D94E, F99Y, F101L, W104Y, Q108R, C109A, A110S, S113E, R116L, I117K, Q121N, I122L, L124I, L127V, N128D, L133R, S136K, L138V, H139R, W140F, S143A, Q145K, R146D, D147K, S148A, S153Q, F161Y, V165T, N170G, T173L, F174Y, M178L, K189N, E190A, T195K, A198P, E199N, L200I, E201K, Q204R, Y205I, R207A, M209D, A211N, L218K, S220D, A221L, R223K, V225I, K226R, A228T, T230V, K231R, M232L, S234A, S239T, S241D, S243Q, I244L, S245M, G252D, E255R, Q256D, Q257V, R258P, C260T, H262W, E265G, E269L, I270R, I272L, S274Q, S275V, C276A, C277I, K281T, I283K, Y284L, T287R, E290N, N292S, T295N, A296M, Y300N, E302C, N303D, D305N, G306S, N307D, T308K, Y312F, M315Q, Q318L, Q319D, N321K, Y322E, R323L, and Y325K, relative to SEQ ID NO:
- 1.
A modified bile salt hydrolase (BSH) enzyme comprising or consisting of one of SEQ ID NOs:
- 46 or 47.
A modified Christensenella minuta bile salt hydrolase (BSH) enzyme with at least 90% identity to SEQ ID NO:
- 1, wherein the modified BSH enzyme has at least one substitution modification relative to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.