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AssigneePeg-Bio Biopharm Co.,Ltd.(Chongqing)
InventorsKai Fan, Yunfeng He, Riyong Liu, Qi Zhang, Guanghui Tang, Tangwei Nie, Qiong Ding, Haiyan Wen, Yanmin Bai, Qing Chen
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Provided are an arginine deiminase mutant, a covalent dimer and a conjugate and a use thereof. The provided arginine deiminase mutant forms a covalent disulfide bond between arginine deiminase subunits by means of mutation of an amino acid site on arginine deiminase. Also provided are a covalent conjugate and a use in the field of cancer treatment. Site-directed mutagenesis of an amino acid site promotes formation of a covalent disulfide bond between non-covalent homodimeric arginine deiminase subunits, and a formed dimer has a more stable structure; in addition, the exposure level of an epitope on an interaction interface can be reduced, the in vivo immunogenicity is reduced, and a more excellent anti-tumor effect is exhibited.
An arginine deiminase mutant, comprising an inter-subunit covalent disulfide bond formed between arginine deiminase subunits via mutation at an amino acid site on arginine deiminase, wherein the mutant exists in the form of a covalent dimer, the covalent dimer formed between non-covalent homodimeric arginine deiminase subunits.
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