Biosynthesis of cannabinoids from cannabigerolic acid using novel cannabinoid synthases
AssigneeNational University of Singapore
InventorsMaybelle Darlene Kho Go, Wen Shan Yew
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Abstract
A method for producing a cannabinoid by contacting cannabigerolic acid with a cannabinoid synthase orthologue. The cannabinoid synthase orthologue is from an organism other than Cannabis sativa. Also disclosed is a recombinant cell of Saccharomyces cerevisiae or Pichia pastoris that includes in its genome a nucleic acid encoding the above cannabinoid synthase orthologue. The cannabinoid synthase orthologue is expressed in the recombinant cell in an active form.
Claims7
A method for producing one or more cannabinoids, the method comprising contacting cannabigerolic acid (CBGA) with a cannabinoid synthase orthologue, wherein the orthologue includes an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 31, 37, 67, 77, 97, 117, and 127.
A recombinant cell of Saccharomyces cerevisiae or Pichia pastoris, comprising in its genome a nucleic acid encoding a cannabinoid synthase orthologue comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 31, 37, 67, 77, 97, 117, and 127, and the cannabinoid synthase orthologue is expressed in the recombinant cell in an active form.
Dependencies are inferred from the claim text.