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AssigneeCytiva BioProcess R&D AB
InventorsJean-Luc Maloisel, Åsa Hagner McWhirter, Ola Lind, Brigitta Németh
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The present disclosure is directed to a chromatography system comprising a buffer valve arrangement configured to allow independent control of a first buffer feed and a second buffer feed; a pump arrangement configured to supply a first buffer feed, a second buffer feed, and a feed comprising a biological target compound and one or more impurities; a selection valve arrangement, comprising a first chromatography device selection valve; a first chromatography device comprising a first chromatography material comprising a support material functionalised with a ligand, wherein the ligand comprises an affinity group having a binding affinity for a biological target compound, an ion exchange group, or a multimodal group; a second chromatography device, comprising a second chromatography material, which comprises a conditioning chromatography material; a third chromatography device comprising a third chromatography material; wherein the selection valve arrangement is configured to enable separation of biological target compounds from impurities by allowing a feed, comprising biological target molecules and one or more impurities, to continuously pass through the first, the second, and the third chromatography devices, wherein the first, the second, and the third chromatography devices are configured to be connected in series. Further provided are a use of the chromatography system and a method for separation of adeno-associated virus capsids from one or more impurities.
A method for separating adeno-associated virus capsids from one or more impurities, said adeno-associated virus capsids being fully packaged with genetic material, said method comprising:
A chromatography system, said system comprising:
Dependencies are inferred from the claim text.