Composite nanofibrous scaffold enhances in vitro hepatocytes' drug clearance profiles to align with human physiology
AssigneeUniversity of Maryland, Baltimore County
InventorsChengpeng CHEN, Rudolph PARK
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Abstract
An in vitro 3D hepatocyte model that contains a nanofibrous scaffold designed to mimic the extracellular matrix (ECM) of the human liver, both structurally and biochemically, is described. HepaRG hepatocytes cultured on the 3D scaffold exhibited enhanced metabolic activity compared to traditional 2D cultures, indicating improved hepatocyte functionality. This model offers a physiologically relevant platform for hepatocyte studies, underscoring the model's potential to advance preclinical drug development by replicating liver-specific functions in vitro.
Claims20
A method of producing a three-dimensional composite nanofibrous scaffold comprising native extracellular matrix (ECM) components, said method comprising:
- electrospinning a polymer-containing solution onto a collector to produce a uniform, three-dimensional layer of nanofibers,
- wherein the polymer-containing solution comprises polycaprolactone (PCL) and pepsinated native decellularized extracellular matrix (dECM) powder, and wherein the nanofibers comprise native ECM components, and wherein the nanofibers comprise native ECM components.
A three-dimensional composite nanofibrous scaffold comprising nanofibers comprising polycaprolactone (PCL) and extracellular matrix (ECM) components of a native tissue.
A three-dimensional tissue, said three-dimensional tissue comprising cells and a composite nanofibrous scaffold comprising nanofibers comprising polycaprolactone (PCL) and extracellular matrix (ECM) components of a native tissue, wherein the cells are present on and/or within the composite nanofibrous scaffold.
Dependencies are inferred from the claim text.