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US 2026/0234558 A1Published application
Methods and Systems for Rapid Engineering of Resting Cells
AssigneeCellfe, Inc.
InventorsChih-Wen Ni, Ailin Goff
Life of this patent
Estimating the term…
Abstract
Non-viral methods and systems for rapidly transfecting resting cells with exogenous material are described. The methods and systems may be used to rapidly transfect cells with multiple components and can be used to manufacture modified immune cells such as resting T-cells.
Claims20
A method for modifying a cell, the method comprising:
- contacting the cell with a first set of components of a genome editing system, wherein the first set of components comprises a guide RNA having a nucleotide sequence capable of hybridizing to a target location, a pegRNA having an attP attachment site, and a nickase or a nucleic acid having a nucleotide sequence encoding the nickase;
- and
- contacting the cell with a second set and a third set of components of a genome editing system, wherein the second set of components comprises a serine-integrase (PhiC31) or a nucleic acid having a nucleotide sequence encoding a serine-integrase (PhiC31), and the third set of components comprises a nucleotide sequence encoding a nucleic acid sequence of interest and an attB site docking site.
Dependencies are inferred from the claim text.