Method for neurological sialadenosis modeling and drug efficacy testing through co-culture of human salivary gland organoids and neurocytomas

A method for preparing a co-culture of a salivary gland organoid and a neuroblastoma, comprising:

• (a) culturing a salivary gland organoid from a salivary gland tissue-derived epithelial cell in a culture medium containing a transforming growth factor-beta inhibitor (TGF-β inhibitor), a Wnt activator, a bone morphogenetic protein (BMP) inhibitor, a fibroblast growth factor (FGF) family, a receptor tyrosine kinase ligand, and a Rho-associated protein kinase (ROCK) inhibitor;
• (b) culturing a neuroblastoma;
• and
• (c) preparing a co-culture by mixing and culturing the salivary gland organoid and the neuroblastoma in a culture medium containing a TGF-β inhibitor, a Wnt activator, a BMP inhibitor, an FGF family, a receptor tyrosine kinase ligand, a ROCK inhibitor, and a cyclic adenosine monophosphate (cAMP) activator.

A method for preparing a neurological sialadenosis model, comprising:

• (a) culturing a salivary gland organoid from a salivary gland tissue-derived epithelial cell in a culture medium containing a transforming growth factor-beta inhibitor (TGF-β inhibitor), a Wnt activator, a bone morphogenetic protein (BMP) inhibitor, a fibroblast growth factor (FGF) family, a receptor tyrosine kinase ligand, and a Rho-associated protein kinase (ROCK) inhibitor;
• (b) culturing a neuroblastoma;
• (c) preparing a co-culture by mixing and culturing the salivary gland organoid and the neuroblastoma in a culture medium containing a TGF-β inhibitor, a Wnt activator, a BMP inhibitor, an FGF family, a receptor tyrosine kinase ligand, a ROCK inhibitor, and a cyclic adenosine monophosphate (cAMP) activator;
• and
• (d) treating the co-culture with a neurotransmitter.