Method for producing microglia-containing cerebral organoid

Abstract

Provided is a method for producing a microglia-containing cerebral organoid, the method comprises: (a) a step for preparing an embryoid body from a pluripotent stem cell; (b) a step for culturing the embryoid body in the presence of a BMP signaling pathway inhibitor and a TGFβ signaling pathway inhibitor, thereby producing a neuralized embryoid body; (c) a step for co-culturing the neuralized embryoid body with a hematopoietic progenitor cell in the presence of an EGF signaling pathway activator, an FGF signaling pathway activator, a TGFβ signaling pathway activator, and an M-CSF signaling pathway activator, thereby producing an initial cerebral organoid; and (d) a step for culturing the initial cerebral organoid in the presence of a TrkB and/or TrkC signaling pathway activator.

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