Injectable off-the- shelf cartilage, tendon, and ligament repair compositions and methods of use
AssigneeEpibone, Inc.
InventorsSarindr BHUMIRATANA, Terri-Ann KELLY, Eric Meade JEFFRIES, Olivia Spencer BEANE, Angela Hai HUANG
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Abstract
Compositions comprising a condensed mesenchymal cell body and a hydrogel are provided. The compositions may further include drugs or growth factors. The condensed mesenchymal cell body may include a connective tissue cell, or even a progenitor cell capable of producing connective tissue extracellular matrices such collagen and glycosaminoglycan. Also provided are methods of treating connective tissue defects, cartilage injury, and cartilage degradation.
Claims14
1-32. (canceled)
An injectable composition for cartilage repair, the composition comprising:
- a) a condensed mesenchymal cell body (CMB) comprising stem cells, wherein:
- i) the stem cells were isolated, passaged once to obtain passage one (P1) stem cells,
- frozen for at least one month, thawed, and passaged to at least passage four (P4) prior to forming the CMB;
- ii) the CMB was frozen at an immature state and thawed prior to administration;
- and
- iii) the immature state is defined as a CMB that has been cultured for fewer than six days prior to freezing;
- and
- b) a hydrogel comprising at least one of collagen, fibrin, or hyaluronic acid.
An injectable composition for cartilage repair, the composition comprising:
- a. a condensed mesenchymal cell body (CMB) comprising stem cells, wherein:
- i. the CMB is generated from between 1,000 and 50,000 cells;
- ii. the CMB was frozen at an immature state;
- and
- iii. the immature state is defined as a CMB that has been cultured for fewer than six days prior to freezing.
Dependencies are inferred from the claim text.