Zymomonas mobilis chassis cell and construction method therefor and use thereof

A method for constructing a Zymomonas mobilis chassis cell, wherein the chassis cell uses Zymomonas mobilis ZMNP-Cas12a with the Cas12a gene integrated at the genomic ZMO0038 locus as a starting strain, integrates the RecET gene into the ZMO0028 locus of the genome of Zymomonas mobilis ZMNP-Cas12a, and the upstream of the RecET gene is linked to a Ptet promoter, and the upstream of the Cas12a gene is linked to a PBAD promoter;

• wherein, the nucleotide sequence of the Cas12a gene is shown in SEQ ID NO.1, the nucleotide sequence of the RecET gene is shown in SEQ ID NO.2, the nucleotide sequence of the Pd promoter is shown in SEQ ID NO.3, and the nucleotide sequence of the PBAD promoter is shown in SEQ ID NO.4;
• the method for constructing the chassis cell comprises the following steps:
• obtaining the upstream homologous arm 0038US and downstream homologous arm 0038DS sequences of the genomic ZMO0038 locus, tandemly linking the PBAD promoter and Cas12a gene between the 0038US and 0038DS sequences, and constructing a first homologous recombination plasmid;
• obtaining the upstream homologous arm 0028US and downstream homologous arm 0028DS sequences of the genomic ZMO0028 locus, tandemly linking the Ptet promoter and RecET gene between the 0028US and 0028DS sequences, and constructing a second homologous recombination plasmid;
• and
• transforming the first homologous recombination plasmid and the second homologous recombination plasmid into Zymomonas mobilis ZMNP-Cas12a respectively, integrating the PBAD promoter and the Cas12a gene into the ZMO0038 locus and integrating the Ptet promoter and the RecET gene into the ZMO0028 locus through homologous recombination, and screening to obtain the Zymomonas mobilis chassis cell.