Chlorella microalgae
AssigneeAlgenuity BV
InventorsAndrew Pearce Spicer, Alexander Findlay Pudney, Aumaya Taleb, Gino Schiano Schiano Di Divisconte, Emma Ransom-Jones, Richard Thomas Smith, Aaron Dickson
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Abstract
Disclosed is a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w/w. Also disclosed is a method of producing a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w/w. Also disclosed is a composition comprising an algae biomass derived from the aforementioned chlorophyll-deficient strain of Chlorella microalgae or obtained by performing aforementioned method.
Claims30
A chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w/w.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having a chlorophyll content in a range of 0.001-0.5 mg/g dry cell weight.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having a protein content in a range of 50-85% w/w.
A chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having a starch content of less than 25% w/w.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having a chitin and/or chitosan, poly-d-glucosamine and poly-acetyl-D-glucosamine content in a range of 0.001 to 4.0 mg/g dry cell weight.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having a protein digestibility-corrected amino acid score (PDCAAS) in a range of 0.75 to 1.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having one or more additional desirable phenotypes, wherein the one or more additional desirable phenotypes is selected from a group comprising:
- a colour, a pigment content, a smell, a taste, a texture, a biochemical composition and improved tolerance to process conditions.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, having a colour selected from at least one of white, cream, pale yellow, yellow, lime, pale green, golden, caramel, orange, pink, red, deep-red, red-brown or brown.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, obtained from a parent strain of Chlorella microalgae, by performing mutagenesis of the parent strain of Chlorella microalgae.
The chlorophyll-deficient strain of Chlorella microalgae of claim 16, wherein the mutagenic chemical is an alkylating agent.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, cultivated in a heterotrophic growth mode.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, cultivated:
- at a specific temperature;
- for a predefined period of time;
- without the presence of light;
- and
- in the presence of an organic carbon energy source.
The chlorophyll-deficient strain of Chlorella microalgae of any preceding claim, being genetically stable.
A method of producing a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w/w, the method comprising:
- a) obtaining a parent strain of Chlorella microalgae;
- b) performing mutagenesis of the parent strain of Chlorella microalgae;
- c) cultivating the mutated strain of Chlorella microalgae at a specific temperature, for a predefined period of time, and in the presence of an organic carbon source;
- and
- d) identifying and isolating chlorophyll-deficient mutants of the parent strain of Chlorella microalgae having a protein content of at least 50% w/w.
A Chlorella microalgae strain selected from the following:
- (i) a Chlorella sorokiniana strain designated CCAP 211/142 deposited under provisions of the Budapest Treaty at the Culture Collection of Algae and Protozoa (CCAP, SAMS Ltd., Scottish Marine Institute, OBAN, Argyll, PA37 1QA, United Kingdom) on Dec. 14, 2023 (Patent Deposit Designation of CCAP 211/142);
- (ii) a Chlorella vulgaris strain designated CCAP 211/143 deposited under provisions of the Budapest Treaty at the Culture Collection of Algae and Protozoa (CCAP, SAMS Ltd., Scottish Marine Institute, OBAN, Argyll, PA37 1QA, United Kingdom) on Dec. 14, 2023 (Patent Deposit Designation of CCAP 211/143).
Dependencies are inferred from the claim text.