3d cell culture system with pumpless perfusion

Abstract

Disclosed herein is a three dimensional (3D) culture system for high-throughput screening with pumpless perfusion. It is a two well flow system where one well has a membrane at the bottom and the other is open. Both wells are connected so the flow is forced in one direction. A dialysis filter membrane can act as a resistor for a system that has a resistance to flow from the 3D culture medium (which is difficult to control) and a resistance from the filter membrane (which is easy to control). The liquid culture media is added to the reservoir well and the 3D culture medium to the sample well. Media will flow through the membrane and the 3D culture medium at a perfusion velocity that is governed in part by the number and size of holes in the membrane.

Dependencies are inferred from the claim text.