Cultureware Slope Features for Seed Cell Concentration

Abstract

Cultureware, such as petri dishes, well plates, tubes, and flasks, are made with a gas permeable bottom having rows of tiny fins configured to trap biological cells in the grooves between them. The tops of the fins can be rounded or slanted such that seed cells that are deposited into the cultureware roll into the grooves. The fins are parallel to each other but can be formed in a wavy, zig-zag, or other undulating pattern. The undulating fin cultureware can be rested on a slanted surface such that deposited seed cells roll into dips formed by the undulations. Footings underneath the resilient bottom that run orthogonal to the grooves can, during centrifugation, promote the formation of temporary local minima in the unsupported areas between the grooves. Seeded cells that are deposited into the cultureware will roll into the local minima when centrifuged and stay congregated with each other when the cultureware is removed from the centrifuge. Devices, methods of manufacture, and use of cultureware with such features are disclosed.

Dependencies are inferred from the claim text.