Purification of capsular polysaccharides

A process of purifying a capsular polysaccharide cell lysate to produce a purified polysaccharide, the process comprising the steps of:

• a) passing the capsular polysaccharide cell lysates through at least one depth filter placed in series resulting in a depth filtered polysaccharide;
• b) introducing the depth filtered polysaccharide onto a first tangential flow filtration (TFF) resulting in a first TTF purified polysaccharide;
• c) precipitating the first TTF purified polysaccharide with a cationic surfactant resulting in a cationic surfactant purified polysaccharide;
• d) mixing the cationic surfactant purified polysaccharide with a solution of potassium iodide (KI) resulting in a KI purified polysaccharide that is in solution;
• e) flowing the KI purified polysaccharide through a carbon filter, wherein the KI purified polysaccharide is loaded at three times load capacity specified for the carbon filter resulting in a carbon filter purified polysaccharide;
• f) flowing the carbon filter purified polysaccharide through a ceramic hydroxyapatite column resulting in a ceramic hydroxyapatite purified polysaccharide, wherein the ceramic hydroxyapatite column is used at a fifty percent load capacity specified for the ceramic hydroxyapatite's column size;
• g) flowing the ceramic hydroxyapatite purified polysaccharide through a hydrophobic interaction (HI) chromatography column resulting in a HI purified polysaccharide;
• and
• h) flowing the HI purified polysaccharide through a second TFF process to obtain a resulting final purified polysaccharide with an overall yield of at least 30%.