Diagnostic system for hemoglobin analysis

A method of identifying and quantifying glycosylated hemoglobin (HbA1c) in a blood sample from a subject, the method comprising:

• providing a diagnostic system that includes a cartridge, wherein the cartridge includes a microchannel extending between first and second buffer pools that each have a buffer solution exhibiting affinity to non-glycosylated hemoglobin, an electrophoresis strip positioned within the microchannel and structured to receive at least a portion of the blood sample, the electrophoresis strip having first and second ends positioned in the first and second buffer pools, respectively, and in fluid communication with the buffer solution in the respective first and second buffer pools, a first electrode exposed to the buffer solution in the first buffer pool;
• a second electrode exposed to the buffer solution in the second buffer pool, the first and second electrodes configured to generate an electric field across the electrophoresis strip;
• collecting the blood sample from the subject;
• depositing the blood sample on the electrophoresis strip;
• and
• applying a voltage across the electrophoresis strip at to cause the separation of the blood sample into various bands of glycosylated hemoglobin and non-glycosylated hemoglobin.