Method for purification of lipid nanoparticles
OwnerKristina SPRINZAR NEMEC, Rok SEKIRNIK +2inventors
InventorsKristina SPRINZAR NEMEC, Rok SEKIRNIK, Andrej RASPOR, Nejc PAVLIN
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Abstract
The present invention relates to methods for the purification of lipid nanoparticles (LNPs) encapsulating a nucleic acid, comprising the steps of subjecting a solution containing said LNPs to a chromatographic medium with convective flow properties in the presence of at least one kosmotropic agent; and eluting LNPs from said chromatographic medium. The present invention further relates to respective uses of a chromatographic medium with convective flow properties for the purification of lipid nanoparticles (LNPs) encapsulating a nucleic acid.
Claims15
A method for the purification of lipid nanoparticles (LNPs) encapsulating a nucleic acid, comprising the steps of:
- (a) subjecting a solution containing said LNPs to a chromatographic medium with convective flow properties in the presence of at least one kosmotropic agent;
- (b) washing the chromatographic medium with a solution containing at least one kosmotropic agent;
- and
- (c) eluting LNPs from said chromatographic medium.
Use of a chromatographic medium with convective flow properties for the purification of lipid nanoparticles (LNPs) encapsulating a nucleic acid, wherein said chromatographic medium is used in the presence of at least one kosmotropic agent.
Dependencies are inferred from the claim text.