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AssigneeThe Regents of the University of California
InventorsRahim Esfandyarpour, Ozdal Boyraz, Jorge Tavares-Negrete, Ceren Babayigit
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As a three-dimensional (3D) bioprinting technique, digital light processing (DLP) has become popular due to its capability to provide high-throughput and high-resolution constructs with precise chemical and biological factor distributions. However, despite the advancements in DLP technology, several hurdles remain, including phototoxicity, extensive printing time, and the limited portfolio of biocompatible/photo-cross-Jinkable materials. Herein, a novel DLP-based 3D-bioprinting technology′ called photopolymerization of orderly-extruded multi-materials (POEM) has been developed and fully characterized. The utility of the POEM technique for rapid and high-resolution 3D-printing of multi-material, multi-layer, and cell-laden structures is demonstrated. The printed configurations showed high cell viability (−80%) and metabolic activity′ for more than five days. The light-based POEM technique described herein enables the fabrication of 3D-cell-laden structures in a multi-material and multi-layer printing manner in biocompatible/photo-cross-linkable materials essential to construct complex heterogeneous tissues/organs.
A method for three-dimensional (3D) bioprinting of multi-layer multi-material structures, the method comprising:
A multi-layer, multi-material, three-dimensional (3D) printed article comprising a hydrogel and living cells, wherein the hydrogel is formed from a pattern of prepolymers comprising a thickening agent, wherein the living cells are incorporated into the pattern of prepolymers, wherein adjacent prepolymers in the pattern of prepolymers differ in prepolymer type, cell type, or a combination thereof.
A three-dimensional (3D) printer cartridge containing a printing solution comprising a plurality of prepolymers having a plurality of prepolymer types, a plurality of living cells having a plurality of cell types, a thickening agent applied to the plurality of prepolymers, and a photo-initiator.
Dependencies are inferred from the claim text.