Substantially Monodisperse Ultrasound Responsive Nanobubble Compositions and Methods of Production and Use Thereof
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsPedram SOUTOUDEHBAGHA, Tara Menon PATTILACHAN, Mehdi RAZAVI
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Abstract
Compositions, methods, and kits relating to substantially monodisperse, ultrasound targeted nanobubble compositions are disclosed. The nanobubble compositions comprise a hollow core containing at least one gas and a polymer shell encircling the hollow core. Methods of producing the substantially monodisperse nanobubble compositions using an ultrasound shearing-based fabrication method are disclosed. Methods of using the nanobubble compositions are also disclosed.
Claims33
A method of preparing a substantially monodisperse, ultrasound responsive targeted nanobubble composition, the method comprising the steps of:
- (1) mixing at least one therapeutic agent with at least one gas and at least one polymer to form a first mixture;
- (2) sonicating and shearing the first mixture to form nanobubbles that comprise a polymer shell formed about a hollow core containing the at least one therapeutic agent and the at least one gas;
- (3) isolating the nanobubbles;
- and
- (4) contacting the nanobubbles with at least one targeting agent to form a second mixture and incubating the second mixture under conditions that allow the at least one targeting agent to be incorporated in the polymer shell and/or attached to a surface of the polymer shell to form the nanobubble composition;
- and
- wherein the nanobubble composition has a polydispersity index value in a range of from about 0.0 to about 0.20.
A substantially monodisperse, ultrasound responsive targeted nanobubble composition, comprising:
- a plurality of nanobubbles that each comprise:
- a hollow core containing at least one gas;
- a polymer shell encircling the hollow core;
- at least one therapeutic agent disposed in the hollow core and/or encapsulated within the polymer shell;
- and
- at least one targeting agent incorporated in the polymer shell and/or attached to a surface of the polymer shell;
- and
- wherein the nanobubble composition has a polydispersity index value in a range of from about 0.0 to about 0.20.
A method, comprising the step of:
- (1) administering an effective amount of at least one ultrasound responsive targeted nanobubble composition to a patient in need thereof, wherein the nanobubble composition comprises a hollow core containing at least one gas, a polymer shell encircling the hollow core, at least one therapeutic agent disposed in the hollow core and/or encapsulated within the polymer shell, and at least one targeting agent incorporated in the polymer shell and/or attached to a surface of the polymer shell, wherein the at least one targeting agent targets the nanobubble composition to at least a portion of a musculoskeletal system of the patient, and wherein the nanobubble composition has a polydispersity index value in a range of from about 0.0 to about 0.20.
Dependencies are inferred from the claim text.