US 2026/0231992 A1 · A PROCESS FOR MYCOTOXIN DETOXIFICATION USING LACTOCOCCUS LACTIS

Abstract

The present invention relates to a method of in-vitro detoxification of multiple mycotoxins using Lactococcus lactis subsp. lactis which is isolated from traditional milk samples. The mycotoxins as disclosed in the present invention are ochratoxin A (OTA), zearalenone (ZEA), fumonisins B1 (FB1), deoxynivalenol (DON) and citrinin (CIT). In one aspect present invention relates to process of inactivation of spore germination and inhibition of biosynthesis of fumonisin B1 micotoxin using Lactococcus lactis subsp. lactis. The species Lactococcus lactis subsp. lactis produces novel antimicrobial metabolites such as 2,4-diotert-butylphenol, Pyrazinamide, and 3-5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one.

Claims15

Dependent claims

1-14. (canceled)

A composition comprising:

(i) live cell pellets (CP) of Lactococcus lactis subsp. lactis having GenBank accession number MG917752;
and
(ii) fermented cell-free supernatant (CFS) comprising one or more metabolites produced by Lactococcus lactis subsp. lactis in a solvent,
wherein the CP of Lactococcus lactis subsp. lactis is in a concentration in the range of 5-15% w/v;
and the fermented cell-free supernatant (CFS) is in a concentration in the range of 5-15% v/v.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

19/157,609Filed 19 Feb 2024

Priority

IN 20231101167820 Feb 2023

Published

13 Aug 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

A23L 5/28
Removal of unwanted matter, e.g. deodorisation or detoxification: using microorganisms
A23L 33/135
Using additives: bacteria or derivatives thereof, e.g. probiotics
A23B 2/783
Organic compounds; Microorganisms; Enzymes: microorganisms; Enzymes
A61K 35/747
Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs: lactobacilli, e.g. L. acidophilus or L. brevis

A process for mycotoxin detoxification using lactococcus lactis

Life of this patent

A process for mycotoxin detoxification using lactococcus lactis

Life of this patent

Abstract

Claims15