Ion signal optimisation

A non-transitory computer-readable medium comprising instructions which, when executed by a computer, cause the computer to carry out a method comprising:

• acquiring from a mass analyser a plurality of mass spectra from a sample, the sample comprising a plurality of ion masses, wherein a voltage value applied to one or more ion lenses is static while each mass spectrum is obtained and the voltage value is varied between different mass spectra;
• determining from the plurality of mass spectra and corresponding voltage applied to one or more ion lenses during acquisition of each mass spectrum, a voltage value providing an optimum ion signal for each of the plurality of ion masses in the sample;
• and
• storing data indicating the ion mass value and corresponding voltage value providing the optimum ion signal for each of the plurality of ion masses in the sample.

A mass spectrometer comprising:

• one or more ion lenses;
• a mass analyser, mass selection device or mass filter;
• a detector;
• and
• means adapted to execute a method that includes:
• acquiring from the mass analyser a plurality of mass spectra from a sample, the sample comprising a plurality of ion masses, wherein a voltage value applied to the one or more ion lenses is static while each mass spectrum is obtained and the voltage value is varied between different mass spectra;
• determining from the plurality of mass spectra and corresponding voltage applied to the one or more ion lenses during acquisition of each mass spectrum, a voltage value providing an optimum ion signal for each of the plurality of ion masses in the sample;
• and
• storing data indicating the ion mass value and corresponding voltage value providing the optimum ion signal for each of the plurality of ion masses in the sample.