Methods and related aspects for the detection of antibodies
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsChao Wang, MD Ashif Ikbal, Seyedsina Mirjalili
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Abstract
Methods of analyzing antibodies in samples are provided. In some embodiments, the methods include contacting a sample with a plurality of plasmonic metal nanoparticles (MNPs) having a viral antigen binding moiety bound to its surface and a viral antigen that comprises two or more epitopes that bind to the viral antigen binding moiety to form a binding composition. In these embodiments, the methods include detecting an extinction signal intensity to identify antibody capabilities in the sample, including the quantity and quality of the antibody. Related systems and other methods are also provided.
Claims20
A method of analyzing an antibody in a sample, the method comprising:
- contacting the sample with a plurality of plasmonic metal nanoparticles (MNPs) having a viral antigen binding moiety bound to its surface and viral antigen that comprises two or more epitopes that bind to the viral antigen binding moiety to form a binding composition;
- and,
- detecting an MNP extinction wavelength from the binding composition, which MNP extinction wavelength is indicative of a level of interaction of the antibody with the viral antigen, thereby analyzing the antibody in the sample.
A method of analyzing a sample, the method comprising:
- contacting the sample with a first plurality of plasmonic metal nanoparticles (MNPs) having a viral antigen bound to its surface and a second plurality of MNPs having a viral antigen binding moiety bound to its surface to form a binding composition, wherein the viral antigen comprises at least one target epitope and wherein the viral antigen binding moiety specifically binds to the target epitope;
- and,
- detecting a first, a second, or a third MNP extinction wavelength from the binding composition, wherein the first MNP extinction wavelength is indicative of the presence of a neutralizing antibody in the sample, wherein the second MNP extinction wavelength is indicative of the presence of a non-neutralizing antibody in the sample and wherein the third MNP extinction wavelength is indicative of the absence of the neutralizing or the non-neutralizing antibody in the sample, thereby analyzing the sample.
A system, comprising:
- a receptacle receiving area configured to receive at least one receptacle that comprises a binding composition comprising a plurality of plasmonic metal nanoparticles (MNPs) having a viral antigen binding moiety bound to its surface and viral antigen that comprises two or more epitopes that bind to the viral antigen binding moiety;
- a light source capable of emitting photons having an MNP extinction wavelength corresponding to the plurality of MNPs;
- and
- a detector capable of detecting transmitted light from the plurality of MNPs.
Dependencies are inferred from the claim text.