Optical measurement method, optical measurement system, and test kit

An optical measurement method using an optical measurement device,

• the optical measurement device including:
• a substrate including an optical waveguide;
• a reaction tank, which is provided on the substrate, and has, at a bottom portion, a detection surface formed by a part of a surface of the optical waveguide;
• a magnetic field application unit configured to apply a magnetic field to magnetic particles dispersed in a sample accommodated in the reaction tank and generate a magnetic force on the magnetic particles in a direction toward the detection surface;
• and
• a light detection unit configured to cause light to enter the optical waveguide, and detect light emitted from the substrate after being reflected by the detection surface,
• the optical measurement method comprising:
• a preparation step of preparing the optical measurement device, the reaction tank in the optical measurement device accommodating the sample containing the magnetic particles on each of which a substance that specifically binds to a target substance is immobilized;
• a magnetic field application step of applying the magnetic field to the magnetic particles by the magnetic field application unit;
• an inspection value acquisition step of acquiring an inspection value based on a amount of light detected by the light detection unit under a state in which the magnetic field is applied;
• and
• a determination step of performing determination of one of presence or absence of the target substance in the sample based on the inspection value.