Single-Molecule Fluorescence Detection System and Method
AssigneeThe University of Sheffield
InventorsTimothy David Craggs, Benjamin Mark Ambrose, Elliot McCallum Steele
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Abstract
A solution-based single-molecule fluorescence spectroscopy system comprising: an excitation source for illuminating an excitation volume of a fluorescently labelled sample solution with excitation radiation; a detector for detecting fluorescence emission radiation emitted from a detection volume of the fluorescently labelled sample solution; one or more processors configured to: receive a detection signal from the detector; calculate a sample signal occupancy representing a proportion of time that the detection signal comprises a sample emission signal from the fluorescently labelled sample solution; and output a sample quality signal based on the sample signal occupancy.
Claims21
A solution-based single-molecule fluorescence spectroscopy system comprising:
- an excitation source for illuminating an excitation volume of a fluorescently labelled sample solution with excitation radiation;
- a detector for detecting fluorescence emission radiation emitted from a detection volume of the fluorescently labelled sample solution;
- one or more processors configured to:
- receive a detection signal from the detector;
- calculate a sample signal occupancy representing a proportion of time that the detection signal comprises a sample emission signal from the fluorescently labelled sample solution;
- and
- output a sample quality signal based on the sample signal occupancy.
A method of determining a sample quality of a fluorescently labelled sample solution in a single-molecule fluorescence spectroscopy system, the method comprising:
- receiving a detection signal from the detector of the system;
- calculating a sample signal occupancy representing a proportion of time that the detection signal comprises a sample emission signal from the fluorescently labelled sample solution;
- and
- outputting a sample quality signal based on the sample signal occupancy.
(canceled)
Dependencies are inferred from the claim text.