Doublet analysis in flow cytometry
AssigneeBeckman Coulter, Inc.
InventorJames CATES
Life of this patent
Estimating the term…
Abstract
A flow cytometry system for analyzing a fluid stream of particles. The system collects waveform data without thresholding radiated light detected from particles passing through a light beam in an interrogation zone. The system identifies doublets from the waveform data. For each doublet identified from the waveform data, the system separates doublet waveforms into individual waveforms. The system analyzes the individual waveforms separated for each doublet and categorizes the doublets based on the analysis of the individual waveforms.
Claims20
A flow cytometry system for analyzing a fluid stream of particles, the flow cytometry system comprising:
- a light source for emitting a light beam toward an interrogation zone;
- an optical system including detectors for detecting radiated light from particles passing through the light beam in the interrogation zone;
- and
- a processing circuitry having non-transitory computer readable storage media storing instructions which, when executed by the processing circuity, cause the processing circuitry to:
- collect waveform data without thresholding the radiated light detected from the particles passing through the light beam in the interrogation zone;
- identify doublets from the waveform data;
- for each doublet identified from the waveform data, separate doublet waveforms into individual waveforms;
- perform an analysis of the individual waveforms separated for each doublet;
- and
- categorize the doublets based on the analysis of the individual waveforms.
A method of analyzing particles flowing through a flow cytometer, the method comprising:
- collecting waveform data without thresholding radiated light detected from the particles passing through a light beam in an interrogation zone;
- identifying doublets from the waveform data;
- for each doublet identified from the waveform data, separating doublet waveforms into individual waveforms;
- analyzing the individual waveforms separated for each doublet;
- and
- categorizing the doublets based on the analysis of the individual waveforms.
A non-transitory computer readable medium comprising program instructions, which when executed by a processor, cause the processor to:
- collect waveform data without thresholding radiated light detected from particles passing through a light beam in an interrogation zone;
- identify doublets from the waveform data;
- for each doublet identified from the waveform data, separate doublet waveforms into individual waveforms;
- perform an analysis of the individual waveforms separated for each doublet;
- and
- categorize the doublets based on the analysis of the individual waveforms.
Dependencies are inferred from the claim text.