Control variable adjustment for flow cytometry waveform acquistion
AssigneeBeckman Coulter, Inc.
InventorsRobert J. ZIGON, Larry MYERS
Life of this patent
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Abstract
A flow cytometer adjusts one or more control variables of a light source, a fluidic system, and an optical system based on a first set of values. The flow cytometer acquires a sequence of waveform data from particles streaming through a light beam in an interrogation zone under the first set of values for the one or more control variables. The flow cytometer adjusts the one or more control variables at predetermined intervals to acquire additional sequences of the waveform data using different sets of values for the one or more control variables.
Claims20
A flow cytometer for analyzing particles, the flow cytometer comprising:
- a light source generating a light beam toward an interrogation zone;
- a fluidic system streaming the particles through the light beam in the interrogation zone;
- an optical system including detectors for detecting radiated light from the particles streaming through the light beam in the interrogation zone;
- and
- a processing circuitry having non-transitory computer readable storage media storing instructions which, when executed by the processing circuity, cause the processing circuitry to:
- adjust one or more control variables of the light source, the fluidic system, and the optical system based on a first set of values;
- acquire a sequence of waveform data from the particles streaming through the light beam in the interrogation zone under the first set of values for the one or more control variables;
- and
- adjust the one or more control variables at predetermined intervals to acquire additional sequences of the waveform data using different sets of values for the one or more control variables.
A method of analyzing particles flowing through an interrogation zone of a flow cytometer, the method comprising:
- adjusting one or more control variables based on a first set of values, the one or more control variables being used to control operation of at least one of:
- a light source generating a light beam toward the interrogation zone;
- a fluidic system causing a flow of the particles through the light beam in the interrogation zone;
- and
- an optical system for detecting one or more characteristics of the particles flowing through the light beam in the interrogation zone;
- acquiring a sequence of waveform data from the particles flowing through the light beam in the interrogation zone under the first set of values for the one or more control variables;
- and
- adjusting the one or more control variables at predetermined intervals to acquire additional sequences of the waveform data using different sets of values for the one or more control variables.
A non-transitory computer readable medium comprising program instructions, which when executed by a processor, cause the processor to:
- adjust one or more control variables based on a first set of values, the one or more control variables being used to control operation of at least one of:
- a light source generating a light beam toward an interrogation zone;
- a fluidic system causing a flow of particles through the light beam in the interrogation zone;
- and
- an optical system for detecting one or more characteristics of the particles flowing through the light beam in the interrogation zone;
- acquire a sequence of waveform data from the particles flowing through the light beam in the interrogation zone under the first set of values for the one or more control variables;
- and
- adjust the one or more control variables at predetermined intervals to acquire additional sequences of the waveform data using different sets of values for the one or more control variables until completion of an experiment duration.
Dependencies are inferred from the claim text.