Kits for genotyping
AssigneesIllumina, Inc., Illumina Cambridge Limited
InventorsAndrew Slatter, Eric Hans Vermaas
Life of this patent
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Abstract
An example of a kit includes a flow cell and a genotyping probe fluid. The flow cell includes a substrate, and first and second capture primers attached to the substrate. The genotyping probe fluid includes a liquid carrier, and a genotyping oligonucleotide in the liquid carrier. The genotyping oligonucleotide includes a first primer sequence; a probe sequence that is representative of a target genotyping locus; a restriction endonuclease site; and a second primer sequence that is at least partially complementary to the second capture primer.
Claims18
-8. (canceled)
A method, comprising:
- introducing a genotyping probe fluid to a flow cell including first and second capture primers, the genotyping probe fluid including a plurality of genotyping oligonucleotides, each of the genotyping oligonucleotides including:
- a first primer sequence;
- a probe sequence representative of a respective target genotyping locus;
- a restriction endonuclease site;
- and
- a second primer sequence that is at least partially complementary to the second capture primer;
- whereby a respective genotyping oligonucleotide reacts to produce respective clonal populations of amplicons from the respective genotyping oligonucleotide;
- linearizing the amplicons to produce probe templates;
- sequencing at least one probe identifying section of the probe templates to identify each of the probe sequences;
- removing at least respective nascent strands from the probe templates, whereby a 3′ OH group at an end of the probe templates is exposed;
- hybridizing respective samples to the probe templates;
- and
- performing respective genotyping reactions of the samples at the exposed 3′ OH groups.
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-26. (canceled)
Dependencies are inferred from the claim text.