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AssigneeYellow Sea Fisheries Research Institute (Ysfri), Chinese Academy of Fishery Sciences (Cafs)
InventorsSheng LUAN, Mianyu LIU, Jie KONG, Qiang FU, Ping DAI, Xianhong MENG, Kun LUO, Juan SUI, Jian TAN, Baolong CHEN, Xupeng LI, Guangfeng QIANG
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The use of an SNP site combination of Litopenaeus vannamei in trait evaluation of mixed-family culture, a probe and a kit. The SNP site combination of Litopenaeus vannamei comprises 1125 SNP sites, wherein the physical positions are determined on the basis of alignment with a reference genome sequence of Litopenaeus vannamei. Also disclosed are a molecular probe for capturing the SNP site combination and a kit comprising the molecular probe. By means of using the SNP site combination for paternity identification, the present invention can achieve the mixed culture of individuals of different families in the early stage, so that the common environmental effect caused by separate family culture in the early stage is effectively reduced, and the accuracy of a trait evaluation result of mixed culture is improved; moreover, the genetic relationship between individuals can be accurately calculated. Compared with physical marks, the present invention avoids the phenomena of falling and dislocation of marks on individuals and human identification errors, thus obtaining accurate pedigrees.
An application of an SNP site combination of Litopenaeus vannamei for use in family mixed breeding trait evaluation, comprising an SNP site combination comprising 1,125 SNP sites, wherein the physical positions of the SNP sites are determined by comparison to the Litopenaeus vannamei reference genome sequence, and wherein information for the SNP sites is shown in Table 1, wherein “ID” is the site identifier, “Scaffold” is the name of the scaffold in the Litopenaeus vannamei reference genome on which the site is located, “Position” is the physical coordinate of the site on the scaffold, and “Ref” is the reference genome base at the site for Litopenaeus vannamei;
Dependencies are inferred from the claim text.