Polymerase for sequencing by synthesis
OwnerRuijie Zhang, Katherine Nakama +1inventors
InventorsRuijie Zhang, Katherine Nakama, Aarushi Grover
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Abstract
Modified polymerases capable of incorporating modified nucleotides are provided. In some aspects, the modified polymerases at least 80% identical to SEQ ID NO: 3 and comprising the following amino acid mutations: an L436 mutation selected from L436Y, L436S, and L436T; a Y437 mutation selected from Y437A and Y437G; and at least one of: a D165A mutation and a E167A mutation. In some aspects, the modified polymerases are used in a sequencing reaction, such as an in situ sequencing reaction.
Claims20
A modified polymerase comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 3 and comprising the following amino acid mutations:
- an L436 mutation selected from L436Y, L436S, and L436T;
- a Y437 mutation selected from Y437A and Y437G;
- and
- at least one of:
- a D165A mutation and a E167A mutation.
Dependencies are inferred from the claim text.