Back to results
US 2026/0226537 A1Published application
Calibration of a nanopore array device
AssigneeOxford Nanopore Technologies PLC
InventorOlle Alfred Nordesjö
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeOxford Nanopore Technologies PLC
InventorOlle Alfred Nordesjö
Estimating the term…
A method of generating a polymer sequence using a nanopore sequencing device, the nanopore array device comprising a nanopore channel, the nanopore channel (35) formed in a membrane (32) separating two ionic solutions (33, 36), the nanopore channel connecting the ionic solutions, the method comprising: translocating a series of polymers through the nanopore channel; generating measurement signals during the translocation of each polymer through the nanopore channel; analysing a set of measurement signals from each polymer taken during the translocation of said polymer through the nanopore channel to determine a scaling factor for each polymer; applying the scaling factor to the measurement signals generated during the translocation of the polymer through the nanopore channel to determine normalised measurement signal values; and generating a polymer sequence for each polymer of the series from the corresponding normalised measurement signal values for each polymer.
A method of generating a polymer sequence using a nanopore sequencing
The method according to any one of the preceding claims, wherein the scaling factors are analysed over the series of polymers to determine the condition of the nanopore channel nanopore device.
The method according to any one of the preceding claims, wherein the nanopore sequencing device comprises an array of nanopore channels, and wherein measurement signals corresponding to the polymers are generated during translocation through the respective nanopore channels.
A method according to any one of the preceding claims, wherein the nanopore is a biological pore.
A method according to any one of the preceding claims, wherein the polymer comprises a series of polymer units to be identified by the nanopore device.
A method according to any one of the preceding claims, wherein a median of the measurement signal value is determined and used in the determination of the normalised measurement signal values.
A method according to any one of the preceding claims, wherein the nanopore device comprises a sensor electrode and the measurements comprise electrical measurements.
The method according to any one of the previous claims, wherein the nanopore device comprises a sensor electrode, and the measurements taken by the sensor are indicative of ion flow through the nanopore.
Dependencies are inferred from the claim text.